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小鼠胚胎成纖維細胞;MEF

更新時間:2016-06-15

簡要描述:

小鼠胚胎成纖維細胞;MEF,開始培養(yǎng)保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關(guān)于人身安全的實驗。

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小鼠胚胎成纖維細胞;MEF
形態(tài)特性 成纖維細胞樣 
生長特性 貼壁生長 
特征特性小鼠胚胎成纖維細胞;MEF取孕9天的615小鼠胚胎,去除腦、心臟等培養(yǎng)建立。該細胞可用作飼養(yǎng)層細胞,支持胚胎干細胞ES的生長并維持ES未分化的狀態(tài)。當作為飼養(yǎng)層細胞時,MEF需經(jīng)絲裂霉素C處理停止生長。建議作為ES細胞的飼養(yǎng)層時,MEF不要超過6代。
培養(yǎng)條件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%CS 
傳代方法 1:3傳代;3~4天1次。
傳代情況 P1
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS 
公司細胞廣泛用于國內(nèi)院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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