更新時間:2016-06-15
人乳腺癌細胞;MCF 7B,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。
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人乳腺癌細胞;MCF 7B
形態特性 上皮樣;梭形
生長特性 貼壁生長
特征特性 人乳腺癌細胞;MCF 7B該細胞由Horoszewicz JS于1977年從一名50歲的明確診斷為轉移性前列腺癌的白人男性患者的左鎖骨上淋巴結細針穿刺的活體組織中分離建立的。5-α-雙氫睪酮可影響該細胞的生長和酸性磷酸酶的產生。該細胞不形成均一的單層而是成簇生長,所以傳代的時候要反復吹打形成單個細胞;該細胞貼壁不牢,達不到匯合狀態,而且會使培養基迅速變酸;傳代后48h內一定要保持培養瓶靜止.如果此時挪動培養瓶,會使大部分細胞從瓶底脫落。如果出現此種情況,需要重新孵育24~48h使細胞重新貼壁,細胞貼壁后可更換培養基。
培養條件 RPMI 1640 (w/o Hepes) 10%FBS
傳代方法 1:3~1:6傳代;5~7天1次。
傳代情況 P50
凍存條件 基礎培養基+5%DMSO+20%FBS
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
分揀連接蛋白15(SNX15)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sorting Nexin 15 (SNX15) X15255 48T/96T Homo sapiens (Human)
分揀連接蛋白1(SNX1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Sorting Nexin 1 (SNX1) X15256 48T/96T Homo sapiens (Human)
連環蛋白α2(CTNNα2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Catenin Alpha 2 (CTNNa2) X15257 48T/96T Homo sapiens (Human)
連環蛋白α3(CTNNα3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Catenin Alpha 3 (CTNNa3) X15258 48T/96T Homo sapiens (Human)
連環蛋白δ1(CTNNδ1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Catenin Delta 1 (CTNNd1) X15259 48T/96T Homo sapiens (Human)
連環蛋白δ2(CTNNδ2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Catenin Delta 2 (CTNNd2) X15260 48T/96T Homo sapiens (Human)
組蛋白脫乙酰基酶3(HDAC3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Histone Deacetylase 3 (HDAC3) X15261 48T/96T Homo sapiens (Human)
組蛋白脫乙酰基酶4(HDAC4)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Histone Deacetylase 4 (HDAC4) X15262 48T/96T Homo sapiens (Human)
組蛋白脫乙酰基酶5(HDAC5)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Histone Deacetylase 5 (HDAC5) X15263 48T/96T Homo sapiens (Human)
組蛋白脫乙酰基酶7(HDAC7)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Histone Deacetylase 7 (HDAC7) X15264 48T/96T Homo sapiens (Human)
組蛋白脫乙酰基酶8(HDAC8)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Histone Deacetylase 8 (HDAC8) X15265 48T/96T Homo sapiens (Human)
組蛋白脫乙酰基酶9(HDAC9)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Histone Deacetylase 9 (HDAC9) X15266 48T/96T Homo sapiens (Human)
組蛋白脫乙酰基酶10(HDAC10)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Histone Deacetylase 10 (HDAC10) X15267 48T/96T Homo sapiens (Human)
組蛋白脫乙酰基酶11(HDAC11)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Histone Deacetylase 11 (HDAC11) X15268 48T/96T Homo sapiens (Human)
沉默調節蛋白3(SIRT3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Sirtuin 3 (SIRT3) X15269 48T/96T Homo sapiens (Human)
沉默調節蛋白5(SIRT5)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Sirtuin 5 (SIRT5) X15270 48T/96T Homo sapiens (Human)
沉默調節蛋白7(SIRT7)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Sirtuin 7 (SIRT7) X15271 48T/96T Homo sapiens (Human)
Vav2癌基因(VAV2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Vav 2 Oncogene (VAV2) X15272 48T/96T Homo sapiens (Human)
Vav3癌基因(VAV3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Vav 3 Oncogene (VAV3) X15273 48T/96T Homo sapiens (Human)
Arkadia蛋白(ARK)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Arkadia (ARK) X15274 48T/96T Homo sapiens (Human)
抑絲蛋白3(PFN3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Profilin 3 (PFN3) X15275 48T/96T Homo sapiens (Human)
抑絲蛋白2(PFN2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Profilin 2 (PFN2) X15276 48T/96T Homo sapiens (Human)
抑絲蛋白4(PFN4)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Profilin 4 (PFN4) X15277 48T/96T Homo sapiens (Human)
剪接因子1(SF1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Splicing Factor 1 (SF1) X15278 48T/96T Homo sapiens (Human)
剪接因子4(SF4)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Splicing Factor 4 (SF4) X15279 48T/96T Homo sapiens (Human)
木糖基轉移酶Ⅰ(XYLT1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Xylosyltransferase I (XYLT1) X15280 48T/96T Homo sapiens (Human)
輸出蛋白7(XPO7)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Exportin 7 (XPO7) X15281 48T/96T Homo sapiens (Human)
輸出蛋白6(XPO6)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Exportin 6 (XPO6) X15282 48T/96T Homo sapiens (Human)
輸出蛋白4(XPO4)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Exportin 4 (XPO4) X15283 48T/96T Homo sapiens (Human)
輸出蛋白3(XPO3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Exportin 3 (XPO3) X15284 48T/96T Homo sapiens (Human)
24-脫氫膽固醇還原酶(DHCR24)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for 24-Dehydrocholesterol Reductase (DHCR24) X15285 48T/96T Homo sapiens (Human)
蛋白O-巖藻糖基轉移酶2(POFUT2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Protein O-Fucosyltransferase 2 (POFUT2) X15286 48T/96T Homo sapiens (Human)
巖藻糖基轉移酶2(FUT2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Fucosyltransferase 2 (FUT2) X15287 48T/96T Homo sapiens (Human)
巖藻糖基轉移酶1(FUT1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Fucosyltransferase 1 (FUT1) X15288 48T/96T Homo sapiens (Human)
巖藻糖基轉移酶11(FUT11)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Fucosyltransferase 11 (FUT11) X15289 48T/96T Homo sapiens (Human)
巖藻糖基轉移酶5(FUT5)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Fucosyltransferase 5 (FUT5) X15290 48T/96T Homo sapiens (Human)
巖藻糖基轉移酶6(FUT6)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Fucosyltransferase 6 (FUT6) X15291 48T/96T Homo sapiens (Human)
巖藻糖基轉移酶7(FUT7)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Fucosyltransferase 7 (FUT7) X15292 48T/96T Homo sapiens (Human)
巖藻糖基轉移酶8(FUT8)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Fucosyltransferase 8 (FUT8) X15293 48T/96T Homo sapiens (Human)
巖藻糖基轉移酶9(FUT9)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Fucosyltransferase 9 (FUT9) X15294 48T/96T Homo sapiens (Human)
氨基酰化酶2(ACY2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Aminoacylase 2 (ACY2) X15295 48T/96T Homo sapiens (Human)
內收蛋白3(ADD3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Adducin 3 (ADD3) X15296 48T/96T Homo sapiens (Human)
內收蛋白2(ADD2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Adducin 2 (ADD2) X15297 48T/96T Homo sapiens (Human)
腺苷酸激酶3(AK3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Adenylate Kinase 3 (AK3) X15298 48T/96T Homo sapiens (Human)
腺苷酸激酶4(AK4)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Adenylate Kinase 4 (AK4) X15299 48T/96T Homo sapiens (Human)
腺苷酸激酶1(AK1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Adenylate Kinase 1 (AK1) X15300 48T/96T Homo sapiens (Human)
外核苷酸焦磷酸酶/磷酸二酯酶3(ENPP3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) X15301 48T/96T Homo sapiens (Human)
堿核蛋白2(BNC2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Basonuclin 2 (BNC2) X15302 48T/96T Homo sapiens (Human)
布皮質素4(BEST4)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Bestrophin 4 (BEST4) X15303 48T/96T Homo sapiens (Human)
布皮質素3(BEST3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Bestrophin 3 (BEST3) X15304 48T/96T Homo sapiens (Human)
布皮質素2(BEST2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Bestrophin 2 (BEST2) X15305 48T/96T Homo sapiens (Human)
鈣蛋白酶6(CAPN6)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Calpain 6 (CAPN6) X15306 48T/96T Homo sapiens (Human)
鈣蛋白酶7(CAPN7)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Calpain 7 (CAPN7) X15307 48T/96T Homo sapiens (Human)
鈣蛋白酶11(CAPN11)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Calpain 11 (CAPN11) X15308 48T/96T Homo sapiens (Human)
鈣磷蛋白2(CAPS2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Calcyphosine 2 (CAPS2) X15309 48T/96T Homo sapiens (Human)
羰基還原酶3(CBR3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Carbonyl Reductase 3 (CBR3) X15310 48T/96T Homo sapiens (Human)
中心體蛋白3(CETN3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Centrin 3 (CETN3) X15311 48T/96T Homo sapiens (Human)
中心體蛋白2(CETN2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Centrin 2 (CETN2) X15312 48T/96T Homo sapiens (Human)
中心體蛋白1(CETN1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Centrin 1 (CETN1) X15313 48T/96T Homo sapiens (Human)
羧酸酯酶2(CES2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Carboxylesterase 2 (CES2) X15314 48T/96T Homo sapiens (Human)
羧酸酯酶3(CES3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Carboxylesterase 3 (CES3) X15315 48T/96T Homo sapiens (Human)
糖合酶3(CHSY3)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Carbohydrate Synthase 3 (CHSY3) X15316 48T/96T Homo sapiens (Human)
封閉蛋白2(CLDN2)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Claudin 2 (CLDN2) X15317 48T/96T Homo sapiens (Human)
封閉蛋白5(CLDN5)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Claudin 5 (CLDN5) X15318 48T/96T Homo sapiens (Human)
封閉蛋白16(CLDN16)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Claudin 16 (CLDN16) X15319 48T/96T Homo sapiens (Human)
封閉蛋白7(CLDN7)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Claudin 7 (CLDN7) X15320 48T/96T Homo sapiens (Human)
封閉蛋白8(CLDN8)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Claudin 8 (CLDN8) X15321 48T/96T Homo sapiens (Human)
封閉蛋白12(CLDN12)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Claudin 12 (CLDN12) X15322 48T/96T Homo sapiens (Human)
封閉蛋白14(CLDN14)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Claudin 14 (CLDN14) X15323 48T/96T Homo sapiens (Human)
Archain 1蛋白(ARCN1)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Archain 1 (ARCN1) X15324 48T/96T Homo sapiens (Human)
羧肽酶A4(CPA4)檢測試劑盒(酶聯免疫吸附試驗法) ELISA Kit for Carboxypeptidase A4 (CPA4) X15325 48T/96T Homo sapiens (Human)
羧肽酶A5(CPA5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase A5 (CPA5) X15326 48T/96T Homo sapiens (Human)