更新時間:2016-06-15
人前列腺癌細胞;LNCaP,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。
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人前列腺癌細胞;LNCaP
形態特性 上皮樣;梭形
生長特性 貼壁生長
特征特性人前列腺癌細胞;LNCaP該細胞由Horoszewicz JS于1977年從一名50歲的明確診斷為轉移性前列腺癌的白人男性患者的左鎖骨上淋巴結細針穿刺的活體組織中分離建立的。5-α-雙氫睪酮可影響該細胞的生長和酸性磷酸酶的產生。該細胞不形成均一的單層而是成簇生長,所以傳代的時候要反復吹打形成單個細胞;該細胞貼壁不牢,達不到匯合狀態,而且會使培養基迅速變酸;傳代后48h內一定要保持培養瓶靜止.如果此時挪動培養瓶,會使大部分細胞從瓶底脫落。如果出現此種情況,需要重新孵育24~48h使細胞重新貼壁,細胞貼壁后可更換培養基。
培養條件 RPMI 1640 (w/o Hepes) 10%FBS
傳代方法 1:3~1:6傳代;5~7天1次。
傳代情況 P50
凍存條件 基礎培養基+5%DMSO+20%FBS
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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