更新時間:2016-06-15
小鼠胚胎成纖維細胞;NIH/3T3,開始培養(yǎng)保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關(guān)于人身安全的實驗。
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小鼠胚胎成纖維細胞;NIH/3T3
形態(tài)特性 成纖維細胞樣
生長特性 貼壁生長
特征特性小鼠胚胎成纖維細胞;NIH/3T3NIH/3T3是從NIH Swiss小鼠胚胎培養(yǎng)物中建立的高度接觸性抑制的連續(xù)傳代細胞株。為了培育在形態(tài)學特征上更適合于進行轉(zhuǎn)化分析的亞株,建立的NIH/3T3細胞株又進行了5輪以上亞克隆。該細胞株對肉瘤病毒的轉(zhuǎn)化灶形成和白血病病毒的繁殖高度敏感,對DNA轉(zhuǎn)化及轉(zhuǎn)染研究十分有用。鼠痘病毒陰性。
培養(yǎng)條件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS
傳代方法 當細胞生長至80%或80%以下匯合時即可傳代,切勿達到匯合;1:3~1:6傳代,每周2次。
傳代情況 C5
凍存條件 基礎培養(yǎng)基+5%DMSO+20%FBS
公司細胞廣泛用于國內(nèi)院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究。
ⅩⅤ型膠原(COL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collagen Type XV (COL15) X13859 48T/96T Mus musculus (Mouse)
細胞外基質(zhì)蛋白1(ECM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Extracellular Matrix Protein 1 (ECM1) X13860 48T/96T Mus musculus (Mouse)
封閉蛋白3(CLDN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Claudin 3 (CLDN3) X13861 48T/96T Rattus norvegicus (Rat)
封閉蛋白5(CLDN5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Claudin 5 (CLDN5) X13862 48T/96T Rattus norvegicus (Rat)
多胺調(diào)節(jié)因子1結(jié)合蛋白1(PMFBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) X13863 48T/96T Homo sapiens (Human)
載脂蛋白O(APOO)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Apolipoprotein O (APOO) X13864 48T/96T Homo sapiens (Human)
纖維鞘相互作用蛋白1(FSIP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fibrous Sheath Interacting Protein 1 (FSIP1) X13865 48T/96T Homo sapiens (Human)
連接附著分子3(JAM3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Junctional Adhesion Molecule 3 (JAM3) X13866 48T/96T Homo sapiens (Human)
連接附著分子1(JAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Junctional Adhesion Molecule 1 (JAM1) X13867 48T/96T Homo sapiens (Human)
中間絲蛋白(SYNC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Syncoilin (SYNC) X13868 48T/96T Homo sapiens (Human)
抵抗素樣蛋白β(RETNLβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Resistin Like Beta (RETNLb) X13869 48T/96T Homo sapiens (Human)
毒蕈堿型膽堿受體M2(CHRM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cholinergic Receptor, Muscarinic 2 (CHRM2) X13870 48T/96T Mus musculus (Mouse)
毒蕈堿型膽堿受體M2(CHRM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cholinergic Receptor, Muscarinic 2 (CHRM2) X13871 48T/96T Rattus norvegicus (Rat)
胸腺基質(zhì)淋巴細胞生成素(TSLP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Thymic Stromal Lymphopoietin (TSLP) X13872 48T/96T Mus musculus (Mouse)
L1-細胞粘附分子(L1CAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for L1-Cell Adhesion Molecule (L1CAM) X13873 48T/96T Mus musculus (Mouse)
天冬酰胺合成酶(ASNS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Asparagine Synthetase (ASNS) X13874 48T/96T Homo sapiens (Human)
白細胞彈性蛋白酶抑制因子(LEI)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Leukocyte Elastase Inhibitor (LEI) X13875 48T/96T Mus musculus (Mouse)
蛋白酪氨酸磷酸酶受體G(PTPRG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Protein Tyrosine Phosphatase Receptor Type G (PTPRG) X13876 48T/96T Homo sapiens (Human) 興奮性氨基酸轉(zhuǎn)運蛋白1(EAAT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Excitatory Amino Acid Transporter 1 (EAAT1) X13877 48T/96T Homo sapiens (Human)
驅(qū)動蛋白家族成員5A(KIF5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Kinesin Family, Member 5A (KIF5A) X13878 48T/96T Homo sapiens (Human)
驅(qū)動蛋白家族成員18A(KIF18A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Kinesin Family, Member 18A (KIF18A) X13879 48T/96T Homo sapiens (Human)
G蛋白偶聯(lián)雌激素受體1(GPER)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER) X13880 48T/96T Homo sapiens (Human)
Bcl2相關(guān)髓細胞白血病序列1(MCL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) X13881 48T/96T Homo sapiens (Human)
IgG-Fc片段結(jié)合蛋白(FcγBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fc Fragment Of IgG Binding Protein (FcgBP) X13882 48T/96T Homo sapiens (Human)
序列相似家族132成員A(FAM132A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Family With Sequence Similarity 132, Member A (FAM132A) X13883 48T/96T Homo sapiens (Human)
形成素結(jié)合蛋白1(FNBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Formin Binding Protein 1 (FNBP1) X13884 48T/96T Homo sapiens (Human)
肌脂蛋白(SLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Sarcolipin (SLN) X13885 48T/96T Homo sapiens (Human)
D-多巴色素變位酶(DDT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for D-Dopachrome Tautomerase (DDT) X13886 48T/96T Homo sapiens (Human)
歸巢關(guān)聯(lián)細胞黏附分子(HCAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Homing Associated Cell Adhesion Molecule (HCAM) X13887 48T/96T Mus musculus (Mouse)
鈣網(wǎng)蛋白(CRT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Calreticulin (CRT) X13888 48T/96T Rattus norvegicus (Rat)
鈣腔蛋白(CALU)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Calumenin (CALU) X13889 48T/96T Rattus norvegicus (Rat)
成纖維細胞生長因子19(FGF19)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fibroblast Growth Factor 19 (FGF19) X13890 48T/96T Rattus norvegicus (Rat)
S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) X13891 48T/96T Rattus norvegicus (Rat)
白介素17F(IL17F)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Interleukin 17F (IL17F) X13892 48T/96T Rattus norvegicus (Rat)
ⅩⅢ型膠原(COL13)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collagen Type XIII (COL13) X13893 48T/96T Homo sapiens (Human)
Ⅶ型膠原(COL7)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collagen Type VII (COL7) X13894 48T/96T Mus musculus (Mouse)
D-多巴色素變位酶(DDT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for D-Dopachrome Tautomerase (DDT) X13895 48T/96T Mus musculus (Mouse)
彈性蛋白酶3B(ELA3B)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Elastase 3B (ELA3B) X13896 48T/96T Rattus norvegicus (Rat)
泛素關(guān)聯(lián)蛋白2(UBAP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Ubiquitin Associated Protein 2 (UBAP2) X13897 48T/96T Homo sapiens (Human)
尿溶蛋白3A(UPK3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Uroplakin 3A (UPK3A) X13898 48T/96T Homo sapiens (Human)
序列相似家族20成員A(FAM20A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Family With Sequence Similarity 20, Member A (FAM20A) X13899 48T/96T Homo sapiens (Human)
半胱氨酰白三烯受體1(CYSLTR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cysteinyl Leukotriene Receptor 1 (CYSLTR1) X13900 48T/96T Homo sapiens (Human)
彈性蛋白酶(ELA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Elastase 2A (ELA2A) X13901 48T/96T Rattus norvegicus (Rat)
碳分解代謝物阻遏4樣蛋白(CCRN4L)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Carbon Catabolite Repression 4 Like Protein (CCRN4L) X13902 48T/96T Homo sapiens (Human)
巰基丙酮酸硫轉(zhuǎn)移酶(MPST)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Mercaptopyruvate Sulfurtransferase (MPST) X13903 48T/96T Mus musculus (Mouse)
賴氨酰氧化酶樣蛋白2(LOXL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lysyl Oxidase Like Protein 2 (LOXL2) X13904 48T/96T Rattus norvegicus (Rat)
谷氨酰胺酶(GLS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Glutaminase (GLS) X13905 48T/96T Homo sapiens (Human)
磷酸二酯酶4D(PDE4D)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Phosphodiesterase 4D, cAMP Specific (PDE4D) X13906 48T/96T Homo sapiens (Human)
糖磺基轉(zhuǎn)移酶9(CHST9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Carbohydrate Sulfotransferase 9 (CHST9) X13907 48T/96T Homo sapiens (Human)
微管關(guān)聯(lián)蛋白4(MAP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Microtubule Associated Protein 4 (MAP4) X13908 48T/96T Rattus norvegicus (Rat)
雙特異性磷酸酶1(DUSP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Dual Specificity Phosphatase 1 (DUSP1) X13909 48T/96T Mus musculus (Mouse)
細胞色素P450家族成員1A2(CYP1A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cytochrome P450 1A2 (CYP1A2) X13910 48T/96T Rattus norvegicus (Rat)
細胞色素P450家族成員3A4(CYP3A4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cytochrome P450 3A4 (CYP3A4) X13911 48T/96T Homo sapiens (Human)
δ樣蛋白4(δLL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Delta Like Protein 4 (dLL4) X13912 48T/96T Rattus norvegicus (Rat)
T-框蛋白3(TBX3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for T-Box Protein 3 (TBX3) X13913 48T/96T Homo sapiens (Human)
胃脂酶(LIPF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lipase, Gastric (LIPF) X13914 48T/96T Homo sapiens (Human)
粘蛋白2(MUC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Mucin 2 (MUC2) X13915 48T/96T Rattus norvegicus (Rat)
纖維蛋白原β(FGβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fibrinogen Beta (FGb) X13916 48T/96T Rattus norvegicus (Rat)
非神經(jīng)元性烯醇化酶(NNE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Enolase, Non Neuronal (NNE) X13917 48T/96T Rattus norvegicus (Rat)
Ⅱ型膠原α1(COL2α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collagen Type II Alpha 1 (COL2a1) X13918 48T/96T Rattus norvegicus (Rat)
C-Mer原癌基因酪氨酸激酶(MERTK)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for C-Mer Proto Oncogene Tyrosine Kinase (MERTK) X13919 48T/96T Homo sapiens (Human)
Janus激酶3(JAK3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Janus Kinase 3 (JAK3) X13920 48T/96T Rattus norvegicus (Rat)
纖維蛋白原γ(FGγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fibrinogen Gamma (FGg) X13921 48T/96T Rattus norvegicus (Rat)
前蛋白轉(zhuǎn)化酶枯草溶菌素9(PCSK9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) X13922 48T/96T Mus musculus (Mouse)
心型脂肪酸結(jié)合蛋白(FABP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) X13923 48T/96T Sus scrofa; Porcine (Pig)
三碘甲狀腺原氨酸(T3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Triiodothyronine (T3) X13924 48T/96T General
補體成分4b(C4b)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Complement Component 4b (C4b) X13925 48T/96T Mus musculus (Mouse)
Rho鳥嘌呤核苷酸交換因子7(ARHGEF7)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) X13926 48T/96T Rattus norvegicus (Rat)
冠蛋白1A(CORO1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Coronin 1A (CORO1A) X13927 48T/96T Rattus norvegicus (Rat)
維生素D(VD)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Vitamin D (VD) X13928 48T/96T General 含銅胺氧化酶3(AOC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Amine Oxidase, Copper Containing 3 (AOC3) X13929 48T/96T Mus musculus (Mouse)
肽酰甘氨酸α酰胺化單氧酶(PAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Peptidylglycine Alpha Amidating Monooxygenase (PAM) X13930 48T/96T Homo sapiens (Human)
海藻糖酶(TREH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Trehalase (TREH) X13931 48T/96T Mus musculus (Mouse)
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