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大鼠垂體瘤細胞;GH3

更新時間:2016-06-16

簡要描述:

大鼠垂體瘤細胞;GH3,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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大鼠垂體瘤細胞;GH3
生長特性 疏松貼壁,有漂浮的細胞簇
特征特性 大鼠垂體瘤細胞;GH3GH3細胞系是由Tashjian AH等在1965年7月從一只7月齡的雌性Wistar-Furth大鼠的垂體腫瘤中分離建立的。GH3細胞系不是直接來源于GH1細胞系的克隆,而是從原代培養的GH1細胞在大鼠身上傳代兩次形成的腫瘤中建立的。上皮樣的GH3細胞比GH1分泌更高水平的生長激素,也可產生催乳素。對調控GH3細胞分泌蛋白類激素的研究表明。
培養條件 F10: Ham's F10 Nutrient Mixture 2.5%FBS+15%HS 
傳代方法 1:2~1:4傳代;每周2~3次。
傳代情況 C5
凍存條件 基礎培養基+5%DMSO+20%FBS 
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究。
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